HPLC Method for Analysis of 3-Iodo-2-propynyl-N-butylcarbamate

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Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of 3-Iodo-2-propynyl-N-butylcarbamate .

3-Iodo-2-propynyl-N-butylcarbamate, also known as idocarb,  has the chemical formula C 8 H 12 I N O 2 . It is typically used as a preservative in paint, coatings, and wood preservatives.  In cosmetics, besides as a preservative, it is also used as a fungicide at low concentrations.

3-Iodo-2-propynyl-N-butylcarbamate can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.

Method Parameters

Mobile PhaseMeCN/H2O – 40/60%
BufferH3PO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV, 200 nm
Class of CompoundsPreservative, Hydrophobic, Ionizable, Zwitterionic
Analyzing Compounds3-Iodo-2-propynyl-N-butylcarbamate

HPLC Column Used

Primesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended