HPLC Method For Analysis Of 2,4,6-Trichloroanisole on Newcrom R1 Column on Alltesta™
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of 2,4,6-Trichloroanisole .
2,4,6-Trichloroanisole ( TCA ) is a fungal metabolite of 2,4,6-Trichlorophenol with the chemical formula C 7 H 5 Cl 3 O . TCA has a distinct musty smell that is most evident when wines develop cork taint. It is formed by the methylation of 2,4,6-trichloropenol. Naturally, it is suspected to be produced when airborne fungi and bacteria are presented with chlorinated phenolic compounds. You can find detailed UV spectra of 2,4,6-Trichloroanisole and information about its various lambda maxima by visiting the following link.
2,4,6-Trichloroanisole can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 70/30% |
|---|---|
| Buffer | None |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds | Haloanisole |
| Analyzing Compounds | 2,4,6-Trichloroanisole |