HPLC Method for Analysis of 2-(Methacryloyloxy)ethyl)trimethylammonium chloride on Primesep 100 Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of (2-(Methacryloyloxy)ethyl)trimethylammonium chloride .
2-(Methacryloyloxy)ethyl)trimethylammonium chloride is a cationic monomer with the chemical formula C 9 H 18 ClNO 2 . It is used primarily to synthesize hydrogels. flocculants, and cationic polymers all of which have a wide variety of uses.
(2-(Methacryloyloxy)ethyl)trimethylammonium chloride can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | H2SO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Quaternary amine |
| Analyzing Compounds | (2-(Methacryloyloxy)ethyl)trimethylammonium chloride |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended