HPLC Method for Separation of 2-Dodecylphenol and 4-Dodecylphenol on Chromni Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Dodecylphenol , 4-Dodecylphenol .
Dodecylphenol is an alkylphenol chemical compound with the formula C 18 H 30 O . It is used primarily in industrial manufacturing of detergents, lubricants, and fuels. It is used as an intermediate in the mining industry as well as in the production of a wide variety of resins.
2-Dodecylphenol , 4-Dodecylphenol can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN |
|---|---|
| Buffer | None |
| Flow Rate | 1.0 ml/min |
| Detection | UV 220 nm |
| LOD* | 1. 34.5 ppb, 2. 35.2 ppb |
| Class of Compounds | Phenol |
| Analyzing Compounds | 2-Dodecylphenol,4-Dodecylphenol |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended