HPLC Method for Analysis of 2-Deoxy-D-Glucose on Primesep S2 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Deoxy-D-Glucose · 2-Deoxy-D-Glucose is an organic compound with the molecular formula C 6 H 12 O 5 . · Properties: Appearance: Typically a white to cream-colored powder or crystalline powder. · Molecular weight: ~164.16 g/mol · Solubility: Soluble in water.
Uses: Being investigated as an anti-cancer agent, an antiviral treatment, and an anticonvulsant, and it has shown promise in reducing oxygen dependence in COVID-19 patients.
2-Deoxy-D-Glucose can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid. Detection is performed using ELSD.
Method Parameters
| Mobile Phase | MeCN – 95% |
|---|---|
| Buffer | Formic Acid – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| LOD | 3.25 ppm |
| Class of Compounds | Deoxy Sugar |
| Analyzing Compounds | 2-Deoxy-D-Glucose |