HPLC Method for Analysis of 2-Aminopyridine on Chromni Column
https://sielc.com/hplc-method-for-analysis-of-2-aminopyridineChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Aminopyridine · 2-Aminopyridine is an synthetic organochlorine compound with the molecular formula C 5 H 6 N 2 . · Properties: Appearance: Typically a white powder or crystals. · Molecular weight: ~94.11 g/mol · Solubility: Soluble in water and alcohol. · Uses: Production of the drugs tenoxicam, sulfapyridine, piroxicam, tripelennamine.
2-Aminopyridine can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN – 70% |
|---|---|
| Buffer | Ammonium Formate – 10mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Tertiary Amine |
| Analyzing Compounds | 2-Aminopyridine |
HPLC Column Used
Chromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended