HPLC Method For Analysis Of 2,3-Dichlorobenzoic Anhydride on Primesep S2 Column

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Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of 2,3-Dichlorobenzoic anhydride .

2,3-Dichlorobenzoic Anhydride is an impurity with the chemical formula C 14 H 6 Cl 4 O 3 . It is formed during the production of Lamotrigine, anticonvulsant used to treat epileptic seizures and Bipolar Disorder. Removing 2,3-Dichlorobenzoic Anhydride is paramount for maximizing Lamotrigine purity. It had no known uses outside of quality control. You can find detailed UV spectra of 2,3-Dichlorobenzoic anhydride and information about its various lambda maxima by visiting the following link.

2,3-Dichlorobenzoic anhydride can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of acetonitrile (MeCN). Detection is performed using UV.

Method Parameters

Mobile PhaseMeCN- 100%
BufferNo
Flow Rate1.0 ml/min
DetectionUV 210 nm
Class of CompoundsAnhydride
Analyzing Compounds2,3-Dichlorobenzoic anhydride

HPLC Column Used

Primesep S2, 4.6 x 250 mm, 5 µm, 100 A, dual ended