HPLC Method for Analysis of Moroxydine on Primesep 100 Column
https://sielc.com/hplc-method-for-analysis-moroxydinesChromatogram
Description
· HPLC Method for Analysis of Moroxydine on Primesep 100 Column by SIELC Technologies
Moroxydine is an antiviral medication with the chemical formula C 6 H 13 N 5 O . It is used in the treatment of influenza. It is believed to work through inhibiting the early stages of viral replication. You can find detailed UV spectra of Moroxydine and information about its various lambda maxima by visiting the following link.
Moroxydine can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 240 nm
Method Parameters
| Mobile Phase | MeCN/H2O 75/25% |
|---|---|
| Buffer | AmFm pH 3.0 – 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 240 nm |
| Samples | 0.5 mg/ml |
| Injection volume | 2 µl |
| LOD* | 8 ppb |
| Class of Compounds | Biguanides |
| Analyzing Compounds | Moroxydine |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended