HPLC Method for Separation of a Mixture of Resorcinol, N,N-Bis (2-hydroxy)-p-phenylenediamine, p-Phenylenediamine and 1-Naphthol on Primesep 100 Column
https://sielc.com/hplc-method-for-analysis-mixtureChromatogram
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Samples | N,N-Bis(2-hydroxy)-p-phenylenediamine (0.7 mg/mL)p-Phenylenediamine (1.3 mg/mL)1-Naphthol (0.18 mg/mL)Resorcinol (0.28 mg/mL) |
| Injection volume | 3 µl |
| Class of Compounds | Phenols, Aromatic amines |
| Analyzing Compounds | Resorcinol,1-Naphthol,N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate,p-Phenylenediamine |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended