HPLC Method for Analysis of Methylprednisolone on Primesep B Column
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Description
· HPLC Method for Analysis of Methylprednisolone on Primesep B Column by SIELC Technologies
Methylprednisolone is a more potent, synthetic, corticosteroid than prednisone with the chemical formula C 22 H 30 O 5 . It is used in various conditions, including severe allergic reactions, inflammatory conditions, and as a part of immunosuppressive regimens. It is a derivative of prednisolone and, like prednisone, belongs to the class of glucocorticoids. You can find detailed UV spectra of Methylprednisolone and information about its various lambda maxima by visiting the following link.
Methylprednisolone can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV 245 nm
Method Parameters
| Mobile Phase | MeCN/H2O – 35% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 245 nm |
| Class of Compounds | Corticosteroids |
| Analyzing Compounds | Methylprednisolone |