HPLC Method for Analysis of Methyl cholate on Primesep B Column
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Description
Methyl cholate is a compound with the chemical formula C 25 H 42 O 5 that belongs to the class of bile acids. Bile acids are substances that are produced by the liver and play a crucial role in the digestion and absorption of dietary fats. Cholate is a primary bile acid, and when methylated, it forms methyl cholate.
It’s important to note that bile acids have physiological roles beyond digestion, including the regulation of cholesterol metabolism and acting as signaling molecules.
Methyl cholate can be retained and analyzed using a Primesep B mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 192 nm
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | H3PO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 192 nm |
| Samples | 2 mg/mL in MeCN/H2O – 50/50% |
| Injection volume | 10 µl |
| LOD* | 0.2 ppm |
| Class of Compounds | bile acids |
| Analyzing Compounds | Methyl cholate |