ELSD HPLC Method for Analysis of Dimethylethanolamine (DMAE or DMEA) on Primesep 100 Column
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Description
HPLC Method for Analysis of Dimethylethanolamine (DMAE or DMEA) on Primesep 100 Column by SIELC Technologies
Dimethylethanolamine (DMAE or DMEA) is a chemical compound often associated with nootropics, skincare, and industrial applications.
Applications: Pharmaceutical and Supplement Use:
Nootropic Effects: DMAE is marketed in dietary supplements for its potential to enhance cognitive function, memory, and mood. It is thought to work by increasing acetylcholine synthesis, a neurotransmitter important for brain activity. Skin Care: DMAE is included in some anti-aging creams and cosmetics, where it is claimed to improve skin firmness and reduce fine lines. Industrial Applications:
Intermediate in Synthesis: Used as a precursor in the production of resins, coatings, and polymers. Corrosion Inhibitor: Used in water treatment and other industrial processes. Research and Laboratory Use:
Common in chemical research as a reagent or intermediate.
Dimethylethanolamine (DMAE or DMEA) can be retained, and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and TFA as a buffer. Detection is carried out using ELSD
Method Parameters
| Mobile Phase | MeCN – 20% |
|---|---|
| Buffer | TFA – 0.2% |
| Flow Rate | 1 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C,with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| LOD* | 10 ppm |
| Class of Compounds | Amino alcohol |
| Analyzing Compounds | Dimethylethanolamine (DMAE or DMEA) |