HPLC Method for Analysis of Clonidine on Primesep B Column
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Description
· HPLC Method for Analysis of Clonidine on Primesep B Column by SIELC Technologies
Clonidine is a medication with the chemical formula C 9 H 9 Cl 2 N 3 . It is primarily prescribed for the treatment of hypertension, especially in cases where other antihypertensive medications have not been effective or well-tolerated. Clonidine is available in various formulations, including tablets, patches, and oral suspension.
Clonidine can be retained, and analyzed using a Primesep B mixed-mode stationary phase column. The analysis utilizes an gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is achieved using UV 290 nm
Method Parameters
| Mobile Phase | Gradient MeCN/H2O – 40-80%, 10 min |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 290 nm |
| Class of Compounds | Sulfonamides, sulphonamides, loop diuretics |
| Analyzing Compounds | Clonidine |
HPLC Column Used
Primesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended