HPLC ELSD Method for Analysis of Choline on Obelisc R Column
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Description
· HPLC Method for Analysis of Choline on Obelisc R Column by SIELC Technologies
Choline is an essential nutrient with the chemical formula C 5 H 14 NO . It plays a crucial role in various physiological processes in the body. It is often grouped with B-vitamins, but it is not officially recognized as a vitamin. Choline serves several important functions, including:
Choline is found in a variety of foods including but not limited to eggs, meat, poultry, and fish, dairy products, nuts and seeds, certain vegetables, such as broccoli and Brussels sprouts
Choline can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium acetate as a buffer. Detection is achieved using ELSD
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | Ammonium acetate pH 5.0 – 10 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 70°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Samples | 1 mg/ml |
| Injection volume | 1 µl |
| LOD* | 200 ppb |
| Class of Compounds | Quaternity amines |
| Analyzing Compounds | Choline |