ELSD HPLC Method for Analysis of Choline on Primesep 100 Column
https://sielc.com/hplc-method-for-analysis-choline-ps100Chromatogram
Description
· HPLC Method for Analysis of Choline on Primesep 100 Column by SIELC Technologies
Choline is an essential nutrient that plays a vital role in many physiological processes in the body. While it is not classified as a vitamin or mineral, it is often grouped with the B-complex vitamins due to its similar functions.
Sources of Choline: Choline can be obtained through diet and is found in a variety of foods: · Rich Sources:
Eggs (especially the yolks) Liver (beef and chicken) Fish (salmon and cod) Soybeans and soy products Moderate Sources:
Broccoli Brussels sprouts Peanuts Whole grains Supplements:
Choline supplements, such as choline bitartrate, phosphatidylcholine, or citicoline, are available for those who may have difficulty getting enough through diet alone.
Method Parameters
| Mobile Phase | MeCN 40% |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 1 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C,with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| LOD* | 3 ppm |
| Class of Compounds | Quaternary ammonium compounds (QACs) |
| Analyzing Compounds | Choline |