HPLC ELSD Method for Analysis Butyrylcholine on Obelisc R Column
https://sielc.com/hplc-method-for-analysis-butyrylcholineChromatogram
Description
· HPLC Method for Analysis of Butyrylcholine on Obelisc R Column by SIELC Technologies
Butyrylcholine is a choline-based ester that can function as a neurotransmitter with the chemical formula C 9 H 20 NO 2 + . It is a synthetic compound similar to acetylcholine. It is used primarily to distinguish between cholinesterases. You can find detailed UV spectra of Butyrylcholine and information about its various lambda maxima by visiting the following link.
Butyrylcholine can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD
Method Parameters
| Mobile Phase | MeCN/H2O – 70/30% |
|---|---|
| Buffer | Ammonium formate pH 3.0 – 2 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C,with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Samples | 1 mg/ml |
| Injection volume | 1 µl |
| LOD* | 60 ppb |
| Class of Compounds | Quaternity amines |
| Analyzing Compounds | Butyrylcholine |
HPLC Column Used
Obelisc R, 2.1 x 100 mm, 5 µm, 100 A, dual ended