HPLC Method for Analysis of Bromophenol blue on Newcrom R1 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromophenol blue
Bromophenol blue (BPB) is a pH indicator and dye commonly used in laboratories, especially in gel electrophoresis and titrations.
pH Indicator Properties Color Changes: Below pH 3.0: Yellow Between pH 3.0 and 4.6: Greenish Above pH 4.6: Blue
Bromophenol blue can be retained and analyzed using the Newcrom R1 column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid as a buffer. Detection is carried out using UV.
You can find detailed UV spectra of Bromophenol blue and information about its various lambda maxima by visiting the following link .
Method Parameters
| Mobile Phase* | Gradient MeCN – 30-70%, 10 min |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | VIS 420, (λmax-200, 280, 424nm) |
| LOD** | 2 ppb |
| Class of Compounds | Dye |
| Analyzing Compounds | Bromophenol blue |
HPLC Column Used
Newcrom R1, 3.2 x 100 mm, 5 µm, 100 A, dual ended