HPLC ELSD Method for Analysis Acetylcholine and Choline on Obelisc R Column
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Description
· HPLC Method for Analysis of Acetylcholine , Choline on Obelisc R Column by SIELC Technologies
Acetylcholine and choline are related compounds that play distinct roles in the body, particularly in the nervous system.
In summary, choline is a nutrient that the body uses to synthesize acetylcholine, among other important molecules. Acetylcholine, on the other hand, is a neurotransmitter responsible for transmitting signals in the nervous system. While choline is obtained from the diet or supplements, acetylcholine is a key mediator of nerve and muscle function in the body.
Acetylcholine and Choline can be retained, separated and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium acetate as a buffer. Detection is achieved using ELSD
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40% |
|---|---|
| Buffer | Ammonium acetate pH 5.0 – 10 mM |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C,with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Samples | 0.5 mg/ml |
| Injection volume | 1 µl |
| LOD* | 200 ppb |
| Class of Compounds | Quaternity amines |
| Analyzing Compounds | Acetylcholine,Choline |