HPLC Method for Analysis of N,N-Bis (2-hydroxy)-p-phenylenediamine on Primesep 100 Column
https://sielc.com/hplc-method-for-analysis-54381-16-7Chromatogram
Description
HPLC Method for Analysis of N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate on Primesep 100 Column by SIELC Technologies
N,N-Bis(2-hydroxyethyl)-p-phenylenediamine , often abbreviated as N,N-Bis(2-hydroxyethyl)-PPD , is a chemical compound with the chemical formula C 10 H 18 N 2 O 6 S . It is a derivative of p-phenylenediamine (PPD), which is a common ingredient in hair dyes.
N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV at 210 nm
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | H2SO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Samples | N,N-Bis(2-hydroxy)-p-phenylenediamine (0.7 mg/mL) |
| Injection volume | 3 µl |
| Class of Compounds | Aromatic amines |
| Analyzing Compounds | N,N-Bis(2-hydroxy)-p-phenylenediamine sulfate |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended