HPLC Method for Analysis of 5-Aminoindol on Primesep 100 Column
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Description
· Separation type: Liquid Chromatography Mixed-mode SIELC Technologies · HPLC Method for Analysis of 5-Aminoindol on Primesep 100 Column
5-Aminoindole is an organic compound with the chemical formula C8H8N2. It is a derivative of indole and features an amino group at the 5-position.
Applications: Pharmaceuticals: It can serve as a building block in the synthesis of various pharmaceutical compounds. Research: Used in studies related to indole derivatives, which are important in medicinal chemistry. 5-Aminoindole be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and sulfuric acid as a buffer. This method allows for detection using UV 200 nm
Method Parameters
| Mobile Phase | MeCN – 55% |
|---|---|
| Buffer | H2SO4 -0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Samples | 1.0 mg/ml MeCN/H2O – 50/50% |
| Injection volume | 1 µl |
| LOD* | 30 ppb (200 nm) |
| Class of Compounds | Pyridines |
| Analyzing Compounds | 5-Aminoindole |