HPLC Chiral Separation of 1-(2-Methylphenyl)-1-pyridin-4-ylmethanamine Enantiomers on Cyralis CM Column
https://sielc.com/hplc-method-for-1-2-methylphenyl-1-pyridin-4-ylmethanamineChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of 1-(2-Methylphenyl)-1-pyridin-4-ylmethanamine dihydrochloride .
1-(2-Methylphenyl)-1-pyridin-4-ylmethanamine has the chemical formula C 13 H 14 N 2 .
This application describes a normal-phase chiral HPLC method for the separation of 1-(2-Methylphenyl)-1-pyridin-4-ylmethanamine dihydrochloride enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.
1-(2-Methylphenyl)-1-pyridin-4-ylmethanamine dihydrochloride can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.
Method Parameters
| Mobile Phase | Hexane/Ethanol/DEA – 70/29/1% |
|---|---|
| Flow Rate | 1.0 mL/min |
| Detection | UV 250 nm |
| Resolution | 3.54 |
| Class of Compounds | Chemical |
| Analyzing Compounds | 1-(2-Methylphenyl)-1-pyridin-4-ylmethanamine dihydrochloride |