HPLC Method for Analysis of Fitusiran on OligoMG Column
https://sielc.com/hplc-method-fitusiranChromatogram
Description
· Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies · HPLC Method for Analyzing Fitusiran on OligoMg Column by SIELC Technologies
Fitusiran is a relatively new therapeutic agent developed for the treatment of hemophilia A and B , including in patients with or without inhibitors.
Using SIELC’s newly introduced BIST™ method, this oligonucleotide can be retained on a OligoMg column. Using this new and unique analysis method, oligonucleotide can be separated, retained, and detected at 260 nm.
Method Parameters
| Mobile Phase | Gradient MeCN 60-10%, 10 min |
|---|---|
| Buffer | none |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm |
| Class of Compounds | Drug, RNA |
| Analyzing Compounds | Fitusiran |
HPLC Column Used
OligoMg, 4.6 x 100 mm, 3 µm, 100 A, dual ended