HPLC Method for Analysis of Biuret on Primesep S Column

Chromatogram

Description

· Separation type: Liquid Chromatography Mixed-mode SIELC Technologies · HPLC Method for Analysis of Biuret on Primesep S Column

Biuret, with the chemical formula C₂H₅N₃O₂, is a white crystalline compound formed by the condensation of two urea molecules with the release of ammonia. Its structure consists of two amide groups (-CONH) linked by a nitrogen atom. Biuret is widely used in biochemistry for protein assays, specifically in the biuret test, which detects peptide bonds. Due to its polar functional groups, the compound is highly soluble in water.

Biuret can be retained, separated and analyzed using a Primesep S mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and phosphoric as a buffer. This method allows for detection using UV 200 nm.

Method Parameters

HPLC Column Used