HPLC Method for Analysis of 6-Aminocaproic Acid in a High Salt Content Matrix on Primesep A Column
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Description
· Separation type: Liquid Chromatography Mixed-mode SIELC Technologies · HPLC Method for Analysis of 6-Aminocaproic acid on Primesep 100 Column · 6-Aminocaproic acid (6ACA) is a synthetic amino acid that is primarily used as a medication.
6-Aminocaproic acid can be retained, separated and analyzed using a Primesep A mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and perchloric acid as a buffer. This method allows for detection using UV 200 nm.
You can find detailed UV spectra of 6-Aminocaproic acid and information about its various lambda maxima by visiting the following link .
Method Parameters
| Mobile Phase | MeCN – 20% |
|---|---|
| Buffer | HClO4 -0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Samples | 1.0 mg/ml in H2O – 100% |
| Injection volume | 10 µl |
| LOD* | 20 ppm (200 nm) |
| Class of Compounds | Acid |
| Analyzing Compounds | 6-Aminocaproic acid |
HPLC Column Used
Primesep A, 4.6 x 250 mm, 5 µm, 100 A, dual ended