HPLC Determination of Carvacrol on Primesep B Column

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Chromatogram

Description

· Separation type: Liquid Chromatography Mixed-mode · HPLC Method for Analysis of Carvacrol on Primesep B Column · High Performance Liquid Chromatography (HPLC) Method for Analysis of Carvacrol

Carvacrol (5-Isopropyl-2-methylphenol) is the main component of the essential oils of the Lamiaceae family of plants. Thymol and carvacrol can also be applied as an alternative antimicrobial agent against antibiotic-resistant pathogenic bacteria.  Carvacrol can be retained in HPLC using Primesep B column with a simple isocratic method using a mobile phase consisting of acetonitrile (MeCN), water and phosphoric acid buffer. The analysis method can be UV detected at 200 nm.

Method Parameters

Mobile PhaseMeCN/H2O – 45/55%
BufferH3PO4 – 0.1%
Flow Rate0.5 ml/min
Detection200 nm
Class of CompoundsPhenol, Hydrophobic
Analyzing CompoundsCarvacrol

HPLC Column Used

[Model]
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