HPLC Determination of Xanthohumol on Newcrom AH Column
https://sielc.com/hplc-determination-of-xanthohumol-on-newcrom-ah-columnChromatogram
Description
Xanthohumol (XN) is a flavonoid compound found in beer that contributes to its flavor and bitterness. There have also been interest in flavonoids and their possible medical applications. Studies have shown that it has anticancer activity and the ability to lower cholesterol and blood sugar. XN can be retained in HPLC on a Newcrom AH mixed-mode column with a mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) as buffer and UV detected at 370nm.
Method Parameters
| Mobile Phase | MeOH/H2O – 70/30% |
|---|---|
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 370 nm |
| Class of Compounds | Hydrophobic, Acid, Chalconoid |
| Analyzing Compounds | Xanthohumol |
HPLC Column Used
Newcrom AH, 4.6×150 mm, 5 µm, 100A