HPLC Determination of Vancomycin on Newcrom R1 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Vancomycin.
Vancomycin is a glycopeptide antibiotic with the chemical formula C 66 H 75 Cl 2 N 9 O 24 . It is used to treat severe but susceptible bacterial infections. Vancomycin is a branched tricyclic glycosylated nonribosomal peptide often reserved as the “drug of last resort”, used only after treatment with other antibiotics has failed. The WHO classifies it as a critically important human medicine, even including it on the List of Essential Medicines.
Vancomycin can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN), water and ammonium formate as a buffer. and detected by UV, ELSD, CAD or LC/MS.
Method Parameters
| Mobile Phase | Gradient MeCN – 5-20%, 10 min |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | 280 nm, CAD |
| Class of Compounds | Drug, Antibiotic |
| Analyzing Compounds | Vancomycin hydrochloride |