HPLC Determination of Valacyclovir on Primesep 100 Column
https://sielc.com/hplc-determination-of-valaciclovirChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Valacyclovir
Valacyclovir belongs to a class of drugs called antiviral drugs and has the chemical formula C 13 H 20 N 6 O 4 . Valacyclovir is used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes as well as shingles. It can also be used to treat chickenpox in children.
Valacyclovir can be retained on a Valacyclovir mixed-mode column with great peak shape using an isocratic method of acetonitrile (ACN), water and sulfuric acid (H2SO4) as a buffer. UV Detection 255nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 255 nm |
| Class of Compounds | Drug |
| Analyzing Compounds | Valacyclovir |
HPLC Column Used
Primesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended