HPLC Determination of Uridine Diphosphate Galactose on Newcrom B Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Uridine 5'-diphosphogalactose disodium salt .
Uridine Diphosphate Galactose ( UDP-galactose ) is an intermediary formed in the biosynthesis of polysaccharides with the chemical formula C 15 H 24 N 2 O 17 P 2 . It is important in glycolysis, which is a metabolic pathway that converts glucose into pyruvate.
UDP-galactose can be detected in the low UV regime. Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate (AmFm) buffer, UDP-galactose can be retained and separated. This analysis method can be UV detected at 265 nm, or via charged aerosol detection (CAD), both with high resolution.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | AmFm – pH 3.0 – 20 mm |
| Flow Rate | 1.0 ml/min |
| Detection | CAD, 262 nm |
| Class of Compounds | Sugar Phosphate |
| Analyzing Compounds | Uridine 5'-diphosphogalactose disodium salt |
HPLC Column Used
Newcrom B, 4.6 x 150 mm, 3 µm, 100 A, dual ended