HPLC Determination of Uridine Diphospate Glucose on Newcrom BH
https://sielc.com/hplc-determination-of-uridine-diphospates-glucose-on-newcrom-bhChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Fructose 1,6 Bisphosphate
Uridine Diphosphate Glucose ( UDP-glucose ) is an an important nucleotide sugar with the chemical formula C 15 H 24 N 2 O 17 P 2 . It is involved in metabolic processes in the cell. It is a precursor to glycogen, UDP-galactose, and UDP-glucuronic acid, as well as other polysacchrides and glycosphingolipids.
UDP-Galactose can be detected in the low UV regime. Using a Newcrom BH mixed-mode column and a mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid (H2SO4) buffer, UDP-galactose can be retained, measured, and analyzed. This analysis method can be UV detected at 210 nm.
Method Parameters
| Mobile Phase | MeCN – 10% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Sugar, Phospate |
| Analyzing Compounds | Uridine-5'-diphosphate-glucose |
HPLC Column Used
Newcrom BH, 2.1 x 150 mm, 3 µm, 100 A, dual ended