HPLC Separation of Uracil, Thymine, Hypoxanthine and Guanine on Newcrom AH
https://sielc.com/hplc-determination-of-uracil-thymine-hypoxanthine-guanineChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analyses of Uracil, Thymine, Hypoxanthine and Guanine
Uracil, Thymine, Hypoxanthine, and Guanine are all nitrogenous bases, each playing distinct roles in the biochemistry of nucleic acids.
Uracil (U) : · Thymine (T) : · Hypoxanthine : · Guanine (G) :
Method Parameters
| Mobile Phase | MeCN – 1% |
|---|---|
| Buffer | Ammonium formate pH 3.0- 6 mM |
| Flow Rate | 1.0 ml/min |
| Peak Retention Time | 2.21, 2.38, 2.81, 7.92 min |
| Detection | 260 nm |
| Class of Compounds | Nucleotides |
| Analyzing Compounds | Uracil,Thymine,Hypoxanthine,Guanine |
HPLC Column Used
Newcrom AH, 4.6 x 150 mm, 5 µm, 100 A, dual ended