HPLC Method for Separation of a Mixture of Tryptophan and its Catabolites on Primesep 100 Column
https://sielc.com/hplc-determination-of-tryptophan-catabolitesChromatogram
Description
· Separation type: Liquid Chromatography Mixed-mode
HPLC Method for Analysis of Mixture of Tryptophan and its Catabolites on Primesep 100 Column by SIELC Technologies
Tryptophan and its catabolites participate in several biological pathways, having roles in protein synthesis, serving as precursors to bioactive molecules, and influencing several physiological processes. Here’s an overview considering a mixture of tryptophan and its catabolites:
.
Tryptophan and its Catabolites can be retained, separated and analyzed on a Primesep 100 mixed-mode stationary phase column using an gradient analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid as a buffer. This analysis method can be detected using UV at 220 nm.
Method Parameters
| Mobile Phase | Gradient MeCN 5-50% in 10 min, with 2 min hold afterwards |
|---|---|
| Buffer | Gradient H2SO4from 0.1-0.2% in 10 min, with 2 min hold afterwards |
| Flow Rate | 1.0 ml/min |
| Detection | UV 220 nm |
| Class of Compounds | Essential Amino AcidTryptophan and its Catabolites |
| Analyzing Compounds | Tryptophan,Picolinic Acid,Kynurenine,3-Hydroxykynurenine,3-Hydroxyanthranilic acid |