HPLC Determination of Tribenzylamine on Primesep B Column
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Description
Tribenzylamine is an organic compound with the chemical formula C 21 H 21 N . It is one of the first compounds produced by the Leuckart reaction. It is primarily used for synthesis of imine.
You can find detailed UV spectra of Tribenzylamine and information about its various lambda maxima by visiting the following link.
Tribenzylamine can be retained and analyzed using the Primesep B stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Formate buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | Gradient MeCN 20-40% , 10 min |
|---|---|
| Buffer | AmFm pH 3.0- 40 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 260 nm, (MS-compatible mobile phase) |
| Class of Compounds | Hydrophobic |
| Analyzing Compounds | Tribenzylamine |
HPLC Column Used
Primesep B, 4.6 x 150 mm, 5 µm, 100 A, dual ended