HPLC Separation of Triazole acetic acid and 1,2,4-Triazole on Primesep 100 Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of 1,2,4-Triazole , Triazole acetic acid
Triazole is a five-membered heterocyclic chemical ring containing three nitrogen atoms and two carbon atoms. It’s molecular formula is C₂H₃N₃ . It is most commonly used as an antifungal drug. It works through inhibiting an enzyme in fungal cell wall synthesis.
1,2,4-Triazole , Triazole acetic acid can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Trifluoroacetic acid buffer. Detection is performed using CAD.
Method Parameters
| Mobile Phase | MeCN/H2O – 25/75% |
|---|---|
| Buffer | TFA – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds | Heterocyclic, Hydrophilic, Base |
| Analyzing Compounds | 1,2,4-Triazole,Triazole acetic acid |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended