HPLC Determination of Thymol on Newcrom R1

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Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Thymol.

Thymol ( 2-isopropyl-5-methylphenol ) is a natural monoterpenoid phenol derivative of p-Cymene. Thymol is the main monoterpene phenol occurring in essential oils isolated from plants such as the Thymus, Ocimum, Origanum, and other herbs with antimicrobial properties.

Thymol can be retained in HPLC using Newcrom R1 column with the simple isocratic mobile phase consisting of methanol (MeOH) and water. The analysis method can be UV detected at 210nm.

Method Parameters

Mobile PhaseMeOH – 70%
BufferNo
Flow Rate0.2 ml/min
DetectionUV 210 nm
Class of CompoundsPhenol, Hydrophobic
Analyzing CompoundsThymol

HPLC Column Used

Newcrom R1, 2.1 x 100 mm, 5 µm, 100 A, dual ended
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