HPLC Determination of Thiocyanate on Newcrom BH Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Potassium Thiocyanate .
Thiocyanate is a pseudohalide with the chemical formula [SCN] − . It works as a biological metabolite in humans and is produced in the liver during detoxification process. While it is a part of the body’s antimicrobial defenses, high presence of it can hinder iodine uptake, leading to an iodine deficiency.
Potassium Thiocyanate can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Acetate buffer. Detection is performed using UV.
Method Parameters
| Mobile Phase | MeCN/H2O – 50/50% |
|---|---|
| Buffer | Ammonium Acetate pH 5.0 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 235 nm, MS-compatible mobile phase |
| Class of Compounds | Anion |
| Analyzing Compounds | Potassium Thiocyanate |
HPLC Column Used
Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended