HPLC Determination of Thiamine Pyrophosphate on PEI Column

Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of Thiamine diphosphate (Thiamine pyrophosphate) .

Thiamine Pyrophosphate , an active form of  Vitamin B1, is a vitamin with the chemical formula C 12 H 19 N 4 O 7 P 2 S + . B1 is a water soluble vitamin with a key function of energy metabolism. It is not produced in the body naturally and is stored in very small amounts; therefore must be regularly consumed through diet of whole rains, nuts, seeds, and legumes. It is vital as a coenzyme in carbohydrate metabolism and in decarboxylation of pyruvate, alpha-ketoglutarate, and branched-chain keto acids.

You can find detailed UV spectra of Thiamine and information about its various lambda maxima by visiting the following link .

Thiamine diphosphate (Thiamine pyrophosphate) can be retained and analyzed using the PEI stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.

Method Parameters

HPLC Column Used