HPLC Determination of Tartrazine (Yellow 5) on Newcrom BH Column
https://sielc.com/hplc-determination-of-tartrazine-on-newcrom-bh-columnChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Tartrazine (FD&C Yellow 5) .
Tartrazine is a yellow food coloring agent. It’s a trisodium salt, and like many multi-charged molecules tends to exhibit significant tailing using reverse-phase HPLC columns. By using a mixed-mode column, specifically designed in mind for multiple charges, Newcrom BH can retain tartrazine with perfect peak shape using a mobile phase of methanol (MeOH) and water with sulfuric acid (H2SO4) to facilitate ion-exchange. UV detection at 430nm.
You can find detailed UV spectra of Yellow 5 and information about its various lambda maxima by visiting the following link.
Method Parameters
| Mobile Phase | MeOH/H2O – 75/25% |
|---|---|
| Buffer | H2SO4 – 1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 430 nm |
| Class of Compounds | Acid, Dyes |
| Analyzing Compounds | Tartrazine (FD&C Yellow 5) |
HPLC Column Used
Newcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended