HPLC Determination of Sunset yellow on Newcrom BH Column

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Chromatogram

Description

· High Performance Liquid Chromatography (HPLC) Method for Analysis of Sunset Yellow (Yellow 6) .

Method Parameters

Mobile PhaseMeOH/H2O – 75/25%
BufferH2SO4 – 0.5%
Flow Rate1.0 ml/min
DetectionVis 480 nm
Class of CompoundsAcid, Dyes
Analyzing CompoundsSunset Yellow (Yellow 6)

HPLC Column Used

Newcrom BH, 4.6 x 50 mm, 5 µm, 100 A, dual ended
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