HPLC Determination of Sulfosuccinic Acid on Newcrom BH Column
https://sielc.com/hplc-determination-of-sulfosuccinic-acidChromatogram
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfosuccinic Acid.
Sulfosuccinic acid is a surfactant with the chemical formula C 4 H 6 O 7 S . It is used as a dispersing agent in pesticides, herbicides, and pigments. In fertilizers, it is used as a complexing agent. It is also used to enhance solubility and bioavailability of drugs in oral dosages.
Sulfosuccinic acid can be retained on the Newcrom BH mixed-mode column using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and sulphuric acid (H2SO4) buffer. The analysis method can be UV detected at 200 nm.
Method Parameters
| Mobile Phase | MeCN/H2O -10/90% |
|---|---|
| Buffer | H2SO4 – 0.3% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Sulfosuccinic acid |
HPLC Column Used
Newcrom BH, 3.2 x 100 mm, 5 µm, 100 A, dual ended