HPLC Determination of Succinic Anhydride and Succinic Acid on SHARC 1 Column

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Chromatogram

Description

High Performance Liquid Chromatography (HPLC) Method for Analysis of Succinic Anhydride and Succinic Acid

Succinic Acid is a dicarboxylic acid with the chemical formula C 4 H 6 O 4 . It is a precursor of some polyesters as well as a component of some alkyd resins. In food, it is typically used as an acidity regulator and flavoring agent. Sulfonic acids like 1-Pentanesulfonic acid, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, and 1=Dodecanesulfonic acid are typically used in ion chromatography and for organic syntheses. You can find detailed UV spectra of Succinic Acid and information about its various lambda maxima by visiting the following link.

Succinic anhydride is a colorless, crystalline compound with the chemical formula C 4 H 4 O 3 . It is used often in the production of polymer resins, especially of the alkyd variety. It has the ability to increase the solubility of various proteins and has other benefits for the pharmaceutical, flavor, and fragrance industries.You can find detailed UV spectra of Succinic anhydride and information about its various lambda maxima by visiting the following link.

Succinic anhydride , Succinic Acid can be retained and analyzed using the SHARC 1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of acetonitrile (MeCN). Detection is performed using UV.

Method Parameters

Mobile PhaseMeCN- 100%*
BufferNo
Flow Rate1.0 ml/min
DetectionUV 210 nm
Class of CompoundsAnhydride, Acid
Analyzing CompoundsSuccinic anhydride,Succinic Acid

HPLC Column Used

SHARC 1, 4.6 x 150 mm, 5 µm, 100 A, dual ended