HPLC Determination of Streptomycin on Primesep 200 Column
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Streptothricins , Streptomycin B , Streptomycin .
Streptomycin is an antibiotic used in treating bacterial infections and has also been used as a pesticide. It is on the World Health Organization’s (WHO) list of essential medicines. It works by inhibiting the synthesis of proteins. It can be retained on a Primesep 200 mixed-mode column by hydrophobic and ion-exchange properties. The analytical method uses MS-compatible mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer. Also capable of UV detection at 200nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 10/90% |
|---|---|
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds | Drug, Hydrophilic, Zwitterionic, Antibiotic |
| Analyzing Compounds | Streptothricins,Streptomycin B,Streptomycin |
HPLC Column Used
Primesep 200