HPLC Determination of Sodium Dodecyl Sulfate on Primesep B4 Column
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HPLC Determination of Sodum Dodecyl Sulfate
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium dodecyl sulfate .
Sodium dodecyl sulfate, or sodium lauryl sulfate, is a surfactant used in cleaning and hygienic products. It has a twelve carbon hydrophobic tail attached to a polar sulfate. Dodecyl sulfate can be retained on Primesep B4 column in under 5 minutes with an MS-compatible mobile phase. Primesep B4 is a short carbon chain reverse-phase (RP) mixed-mode column with embedded basic ion-pairing groups.
Method Parameters
| Mobile Phase | MeCN/H2O – 60/40% |
|---|---|
| Buffer | AmFm pH 3.0 – 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds | Hydrophobic, Base, Ionizable, Anionic surfactant |
| Analyzing Compounds | Sodium dodecyl sulfate |
HPLC Column Used
Primesep B4