HPLC Determination of Sodium Dodecyl Sulfate on Newcrom BH and Newcrom B Columns

Chromatogram

Description

Sodium dodecyl sulfate (SDS) , or sodium lauryl sulfate (SLS), is a surfactant used in cleaning, hygienic, and food products. It has a chemical formula C 12 H 25 NaSO 4 , meaning it has twelve carbon hydrophobic tail attached to a polar sulfate. At low concentrations, it is used as a whipping aid and emulsifier in foods containing egg whites. It has a similar use in pharmaceutics as an ionic solubilizer and, also, an emulsifier. In laboratory uses, it is used as a component for lysing cells during RNA extraction or DNA extraction. You can find detailed UV spectra of Sodium dodecyl sulfate and information about its various lambda maxima by visiting the following link.

Sodium dodecyl sulfate can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.

Method Parameters

HPLC Column Used