HPLC Method for Analysis of Sodium 3-chloro-2-hydroxypropane-1-sulfonate on Newcrom BH
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Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium 3-chloro-2-hydroxypropane-1-sulfonate .
Sodium 3-chloro-2-hydroxypropane-1-sulfonate is an anionic agent with the chemical formula C 3 H 6 ClNaO 4 S . It is used to prepare negatively charged probe particles to investigate the nanoporosity of cellulosic fibers and their interactions with cationic polyelectrolytes.
Sodium 3-chloro-2-hydroxypropane-1-sulfonate can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an Ammonium Formate buffer. Detection is performed using ELSD
Method Parameters
| Mobile Phase | MeOH/H2O – 10/90% |
|---|---|
| Buffer | Ammonium Formate pH 3.0 – 40 mM |
| Flow Rate | 0.5 ml/min |
| Detection | ELSD |
| Class of Compounds | Sulfonate |
| Analyzing Compounds | Sodium 3-chloro-2-hydroxypropane-1-sulfonate |
HPLC Column Used
Newcrom BH, 3.2 x 100 mm, 3 µm, 100 A, dual ended