HPLC Method for Analysis of Sildenafil Citrate (Viagra) in a Tablet Primesep 100 Column
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Description
Sildenafil Citrate (also known as Viagra ) is one of the most popular medications used to treat erectile dysfunction and pulmonary hypertension. It works primarily through increasing bloodflow. It has the chemical formula C 28 H 38 N 6 O 11 S . It is a part of the group called phosphodiesterase 5 (PDE5) inhibitors, which prevent the enzyme called “phosphodiesterase type-5” from working.
Sildenafil (Viagra) can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 214 nm and 294 nm with high resolution and peak symmetry.
Method Parameters
| Mobile Phase | MeCN – 60% |
|---|---|
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | λmax 214, 294 nm |
| Peak Retention Time | 5.7 min |
| Class of Compounds | Drug |
| Analyzing Compounds | Sildenafil (Viagra) |
HPLC Column Used
Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended