HPLC Determination of Seleno Amino Acids on Newcrom AH Column
https://sielc.com/hplc-determination-of-seleno-amino-acidsChromatogram
Description
High Performance Liquid Chromatography (HPLC) Method for Analysis of Seleno-L-cystine , Selenomethionine , Methylselenocysteine .
Methylselenocysteine is used in chemotherapy to induce apoptosis (cell death) of the cancerous cells. Methylselenocysteine is an amino acid with antioxidant properties and a main source of selenium in several foods. Selenocysteine is an analog of cysteine with the selenium replacing sulfur. It has been used as a radioactive tracer. All three analytes are zwitterions and have similar structures which can present difficulties in reversed-phase separation in HPLC. By using a mixed mode Newcrom AH column which has ion-exchange properties in addition to hydrophobic properties allows the separation of the seleno amino acids with an MS-compatible mobile phase of water and acetonitrile (ACN) with formic acid buffer.
Method Parameters
| Mobile Phase | MeCN – 20% |
|---|---|
| Buffer | Formic Acid – 4% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Seleno-L-cystine,Selenomethionine,Methylselenocysteine |