HPLC Determination of Ritodrine on Primesep 100
https://sielc.com/hplc-determination-of-ritodrineChromatogram
HPLC Determination of Ritodrine_1181
Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Ritodrine
Ritodrine (also Yutopar), is a drug used in relaxation of smooth muscle and to stop premature labor. It has been pulled from the US market due to its side effects. Ritodrine can be retained on a Primesep 100 column with great peak shape using isocratic method of 30/70 Acetonitrile (ACN) and water with 0.3% sulfuric acid as buffer. UV Detection 276nm.
Method Parameters
| Mobile Phase | MeCN/H2O – 30/70% |
|---|---|
| Buffer | H2SO4 – 0.3 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 276 nm |
| Class of Compounds | Drug, Basic, Hydrophobic, Ionizable, Amine |
| Analyzing Compounds | Ritodrine |
HPLC Column Used
Primesep 100, 4.6×150 mm, 5 µm, 100A