HPLC Determination of Rifampicin on Newcrom A column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Rifampicin .
Rifampicin is an antibiotic used alongside other antibiotics to treat several types of bacterial infections like tuberculosis and Legionnaires’ disease. It works by inhibiting the bacteria’s RNA production and is on the World Health Organisation’s (WHO) List of essential medicines. It’s chemical formula is C 43 H 58 N 4 O 12 .
It can be retained in HPLC on Newcrom A mixed-mode column. The retention characteristics can be controlled by the organic modifier used in the mobile phase, with methanol (MeOH) having a longer retention time in comparison to acetonitrile (ACN). UV detection at 275nm.
Method Parameters
| Mobile Phase | MeOH, MeCN |
|---|---|
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Hydrophobic, Drugs, Antibiotics |
| Analyzing Compounds | Rifampicin |
HPLC Column Used
Newcrom A, 4.6 x 50 mm, 3 µm, 100 A, dual ended