HPLC Determination of Ractopamine on Primesep 100 Column
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Description
· High Performance Liquid Chromatography (HPLC) Method for Analysis of Ractopamine hydrochloride .
Ractopamine is a livestock feed additive designed to promote muscle synthesis and leanness. It is banned in many countries, excluding the US, Canada and Mexico. It can be retained on a Primesep 100 Column in about 7 minutes. Primesep 100 is a reverse-phase (RP) mixed-mode column with embedded acidic ion-pairing groups.
Method Parameters
| Mobile Phase | MeCN/H2O – 20/80% |
|---|---|
| Buffer | H2SO4 – 0.3 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 222 nm |
| Class of Compounds | Drug, Basic, Hydrophobic, Ionizable, Amine |
| Analyzing Compounds | Ractopamine hydrochloride |
HPLC Column Used
Primesep 100